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cd20 car expression plasmids  (Promega)

 
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    Promega cd20 car expression plasmids
    NFAT-Luc2 activity stimulated by multiple T-cell activation signals. (a) Jurkat/NFAT-Luc2 cells were incubated with α-CD3 antibody crosslinked with goat α-mouse IgG antibody at the indicated concentrations. (b) Jurkat/NFAT-Luc2 cells were incubated with or without Raji target cells and the indicated concentrations of blinatumomab. The curves were compared using an extra-sum-of-squares F test and were determined to be different ( P > .0001) (c) Jurkat/NFAT-Luc2 cells were transiently transfected with varying concentrations of <t>α-CD19-CAR</t> or <t>α-CD20-CAR</t> plasmid DNA, balanced with carrier DNA (pGEM-3z) such that all conditions received the same total amount of DNA. One day after transfection, cells were incubated with or without Raji target cells. In all panels, assays were incubated for 6 h at 37°C, and luciferase activity was detected using the Bio-Glo™ Luciferase Assay System. Data are representative of at least three independent experiments with n = 3 technical replicates. Error bars indicate standard deviation. For (c), a one-way ANOVA was performed separately for a-CD19 and a-CD20 conditions. Dunnett’s multiple comparison post-testing found a significant difference between the carrier DNA only (0:1) condition and the CAR DNA conditions in the presence of Raji cells ( P < .0001) and no differences in the absence of Raji cells. Post-testing for linear trend found a relationship between CAR DNA quantity and assay response in the presence of Raji cells ( P < .0001).
    Cd20 Car Expression Plasmids, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd20 car expression plasmids/product/Promega
    Average 90 stars, based on 1 article reviews
    cd20 car expression plasmids - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "A bioluminescent reporter bioassay for in-process assessment of chimeric antigen receptor lentiviral vector potency"

    Article Title: A bioluminescent reporter bioassay for in-process assessment of chimeric antigen receptor lentiviral vector potency

    Journal: Antibody Therapeutics

    doi: 10.1093/abt/tbae032

    NFAT-Luc2 activity stimulated by multiple T-cell activation signals. (a) Jurkat/NFAT-Luc2 cells were incubated with α-CD3 antibody crosslinked with goat α-mouse IgG antibody at the indicated concentrations. (b) Jurkat/NFAT-Luc2 cells were incubated with or without Raji target cells and the indicated concentrations of blinatumomab. The curves were compared using an extra-sum-of-squares F test and were determined to be different ( P > .0001) (c) Jurkat/NFAT-Luc2 cells were transiently transfected with varying concentrations of α-CD19-CAR or α-CD20-CAR plasmid DNA, balanced with carrier DNA (pGEM-3z) such that all conditions received the same total amount of DNA. One day after transfection, cells were incubated with or without Raji target cells. In all panels, assays were incubated for 6 h at 37°C, and luciferase activity was detected using the Bio-Glo™ Luciferase Assay System. Data are representative of at least three independent experiments with n = 3 technical replicates. Error bars indicate standard deviation. For (c), a one-way ANOVA was performed separately for a-CD19 and a-CD20 conditions. Dunnett’s multiple comparison post-testing found a significant difference between the carrier DNA only (0:1) condition and the CAR DNA conditions in the presence of Raji cells ( P < .0001) and no differences in the absence of Raji cells. Post-testing for linear trend found a relationship between CAR DNA quantity and assay response in the presence of Raji cells ( P < .0001).
    Figure Legend Snippet: NFAT-Luc2 activity stimulated by multiple T-cell activation signals. (a) Jurkat/NFAT-Luc2 cells were incubated with α-CD3 antibody crosslinked with goat α-mouse IgG antibody at the indicated concentrations. (b) Jurkat/NFAT-Luc2 cells were incubated with or without Raji target cells and the indicated concentrations of blinatumomab. The curves were compared using an extra-sum-of-squares F test and were determined to be different ( P > .0001) (c) Jurkat/NFAT-Luc2 cells were transiently transfected with varying concentrations of α-CD19-CAR or α-CD20-CAR plasmid DNA, balanced with carrier DNA (pGEM-3z) such that all conditions received the same total amount of DNA. One day after transfection, cells were incubated with or without Raji target cells. In all panels, assays were incubated for 6 h at 37°C, and luciferase activity was detected using the Bio-Glo™ Luciferase Assay System. Data are representative of at least three independent experiments with n = 3 technical replicates. Error bars indicate standard deviation. For (c), a one-way ANOVA was performed separately for a-CD19 and a-CD20 conditions. Dunnett’s multiple comparison post-testing found a significant difference between the carrier DNA only (0:1) condition and the CAR DNA conditions in the presence of Raji cells ( P < .0001) and no differences in the absence of Raji cells. Post-testing for linear trend found a relationship between CAR DNA quantity and assay response in the presence of Raji cells ( P < .0001).

    Techniques Used: Activity Assay, Activation Assay, Incubation, Transfection, Plasmid Preparation, Luciferase, Standard Deviation, Comparison



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    cd20 car expression plasmids  (Promega)
    90
    Promega cd20 car expression plasmids
    NFAT-Luc2 activity stimulated by multiple T-cell activation signals. (a) Jurkat/NFAT-Luc2 cells were incubated with α-CD3 antibody crosslinked with goat α-mouse IgG antibody at the indicated concentrations. (b) Jurkat/NFAT-Luc2 cells were incubated with or without Raji target cells and the indicated concentrations of blinatumomab. The curves were compared using an extra-sum-of-squares F test and were determined to be different ( P > .0001) (c) Jurkat/NFAT-Luc2 cells were transiently transfected with varying concentrations of <t>α-CD19-CAR</t> or <t>α-CD20-CAR</t> plasmid DNA, balanced with carrier DNA (pGEM-3z) such that all conditions received the same total amount of DNA. One day after transfection, cells were incubated with or without Raji target cells. In all panels, assays were incubated for 6 h at 37°C, and luciferase activity was detected using the Bio-Glo™ Luciferase Assay System. Data are representative of at least three independent experiments with n = 3 technical replicates. Error bars indicate standard deviation. For (c), a one-way ANOVA was performed separately for a-CD19 and a-CD20 conditions. Dunnett’s multiple comparison post-testing found a significant difference between the carrier DNA only (0:1) condition and the CAR DNA conditions in the presence of Raji cells ( P < .0001) and no differences in the absence of Raji cells. Post-testing for linear trend found a relationship between CAR DNA quantity and assay response in the presence of Raji cells ( P < .0001).
    Cd20 Car Expression Plasmids, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd20 car expression plasmids/product/Promega
    Average 90 stars, based on 1 article reviews
    cd20 car expression plasmids - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    NFAT-Luc2 activity stimulated by multiple T-cell activation signals. (a) Jurkat/NFAT-Luc2 cells were incubated with α-CD3 antibody crosslinked with goat α-mouse IgG antibody at the indicated concentrations. (b) Jurkat/NFAT-Luc2 cells were incubated with or without Raji target cells and the indicated concentrations of blinatumomab. The curves were compared using an extra-sum-of-squares F test and were determined to be different ( P > .0001) (c) Jurkat/NFAT-Luc2 cells were transiently transfected with varying concentrations of α-CD19-CAR or α-CD20-CAR plasmid DNA, balanced with carrier DNA (pGEM-3z) such that all conditions received the same total amount of DNA. One day after transfection, cells were incubated with or without Raji target cells. In all panels, assays were incubated for 6 h at 37°C, and luciferase activity was detected using the Bio-Glo™ Luciferase Assay System. Data are representative of at least three independent experiments with n = 3 technical replicates. Error bars indicate standard deviation. For (c), a one-way ANOVA was performed separately for a-CD19 and a-CD20 conditions. Dunnett’s multiple comparison post-testing found a significant difference between the carrier DNA only (0:1) condition and the CAR DNA conditions in the presence of Raji cells ( P < .0001) and no differences in the absence of Raji cells. Post-testing for linear trend found a relationship between CAR DNA quantity and assay response in the presence of Raji cells ( P < .0001).

    Journal: Antibody Therapeutics

    Article Title: A bioluminescent reporter bioassay for in-process assessment of chimeric antigen receptor lentiviral vector potency

    doi: 10.1093/abt/tbae032

    Figure Lengend Snippet: NFAT-Luc2 activity stimulated by multiple T-cell activation signals. (a) Jurkat/NFAT-Luc2 cells were incubated with α-CD3 antibody crosslinked with goat α-mouse IgG antibody at the indicated concentrations. (b) Jurkat/NFAT-Luc2 cells were incubated with or without Raji target cells and the indicated concentrations of blinatumomab. The curves were compared using an extra-sum-of-squares F test and were determined to be different ( P > .0001) (c) Jurkat/NFAT-Luc2 cells were transiently transfected with varying concentrations of α-CD19-CAR or α-CD20-CAR plasmid DNA, balanced with carrier DNA (pGEM-3z) such that all conditions received the same total amount of DNA. One day after transfection, cells were incubated with or without Raji target cells. In all panels, assays were incubated for 6 h at 37°C, and luciferase activity was detected using the Bio-Glo™ Luciferase Assay System. Data are representative of at least three independent experiments with n = 3 technical replicates. Error bars indicate standard deviation. For (c), a one-way ANOVA was performed separately for a-CD19 and a-CD20 conditions. Dunnett’s multiple comparison post-testing found a significant difference between the carrier DNA only (0:1) condition and the CAR DNA conditions in the presence of Raji cells ( P < .0001) and no differences in the absence of Raji cells. Post-testing for linear trend found a relationship between CAR DNA quantity and assay response in the presence of Raji cells ( P < .0001).

    Article Snippet: The NFAT-Luc2 reporter plasmid and CD19 and CD20 CAR expression plasmids were generated at Promega Corp. LV particles were generated by VectorBuilder.

    Techniques: Activity Assay, Activation Assay, Incubation, Transfection, Plasmid Preparation, Luciferase, Standard Deviation, Comparison